pires hrgfp ii tet1 cd Search Results


pires hrgfp ii tet1 cd  (Addgene inc)
93
Addgene inc pires hrgfp ii tet1 cd
Pires Hrgfp Ii Tet1 Cd, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pires+hrgfp+ii+tet1+cd/pmc12081649-16-0-3?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pires hrgfp ii tet1 cd - by Bioz Stars, 2026-07
93/100 stars
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tet1 overexpression vector pires hrgfpii tet1  (Addgene inc)
93
Addgene inc tet1 overexpression vector pires hrgfpii tet1
MSTN mutant demethylates myogenesis-specific genes by up-regulating demethylase <t>TET1</t> . (A, B) Methylation levels of myogenesis-specific genes of MyoD , MyoG , PAX3 , and PAX7 in promoters (A) and gene bodies (B). (C) Expression of demethylase TET1 , TET2 , and TET3 at the mRNA levels. (D, E) Expression of demethylase TET1 at the protein level. *P<0.05, **P<0.01, ***P<0.001; t-tests were used to calculate the p-values.
Tet1 Overexpression Vector Pires Hrgfpii Tet1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pires+hrgfp+ii+tet1+cd/pmc07085230-157-1-9?v=Addgene+inc
Average 93 stars, based on 1 article reviews
tet1 overexpression vector pires hrgfpii tet1 - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier

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MSTN mutant demethylates myogenesis-specific genes by up-regulating demethylase TET1 . (A, B) Methylation levels of myogenesis-specific genes of MyoD , MyoG , PAX3 , and PAX7 in promoters (A) and gene bodies (B). (C) Expression of demethylase TET1 , TET2 , and TET3 at the mRNA levels. (D, E) Expression of demethylase TET1 at the protein level. *P<0.05, **P<0.01, ***P<0.001; t-tests were used to calculate the p-values.

Journal: International Journal of Biological Sciences

Article Title: MSTN Mutant Promotes Myogenic Differentiation by Increasing Demethylase TET1 Expression via the SMAD2/SMAD3 Pathway

doi: 10.7150/ijbs.40551

Figure Lengend Snippet: MSTN mutant demethylates myogenesis-specific genes by up-regulating demethylase TET1 . (A, B) Methylation levels of myogenesis-specific genes of MyoD , MyoG , PAX3 , and PAX7 in promoters (A) and gene bodies (B). (C) Expression of demethylase TET1 , TET2 , and TET3 at the mRNA levels. (D, E) Expression of demethylase TET1 at the protein level. *P<0.05, **P<0.01, ***P<0.001; t-tests were used to calculate the p-values.

Article Snippet: The TET1 overexpression vector pIRES-hrGFPII-TET1 (#83568) was purchased from Addgene.

Techniques: Mutagenesis, Methylation, Expressing

MSTN regulates TET1 expression via SMAD2/SMAD3. (A) The binding region of SMAD2/SMAD3 with the promoter of TET1 . (B) The combination of TET1 promoter with SMAD2/SMAD3 detected by ChIP-qPCR. The detected binding region was from ‒1613 to ‒1437 on the TET1 promoter. (C) Luciferase reporter assays. HEK293T cells were transfected with luciferase reporter plasmids containing the promoter of TET1 (1800 bp). *P<0.05, **P<0.01, ***P<0.001; t-tests were used to calculate the p-values.

Journal: International Journal of Biological Sciences

Article Title: MSTN Mutant Promotes Myogenic Differentiation by Increasing Demethylase TET1 Expression via the SMAD2/SMAD3 Pathway

doi: 10.7150/ijbs.40551

Figure Lengend Snippet: MSTN regulates TET1 expression via SMAD2/SMAD3. (A) The binding region of SMAD2/SMAD3 with the promoter of TET1 . (B) The combination of TET1 promoter with SMAD2/SMAD3 detected by ChIP-qPCR. The detected binding region was from ‒1613 to ‒1437 on the TET1 promoter. (C) Luciferase reporter assays. HEK293T cells were transfected with luciferase reporter plasmids containing the promoter of TET1 (1800 bp). *P<0.05, **P<0.01, ***P<0.001; t-tests were used to calculate the p-values.

Article Snippet: The TET1 overexpression vector pIRES-hrGFPII-TET1 (#83568) was purchased from Addgene.

Techniques: Expressing, Binding Assay, ChIP-qPCR, Luciferase, Transfection

Overexpression of TET1 promotes myogenic differentiation. (A-C) The expression of TET1 at the mRNA and protein level after the overexpression of TET1 in the wild type muscle satellite cells. (D, E) Methylation levels of myogenesis-specific genes in the promoter and gene body. (F) Expression of myogenic genes at the mRNA level. (G, H) Expression of myogenic genes at the protein level. (I) The differentiated myotubes. (J) Myotube fusion index after the overexpression of TET1 . (K) Length of myotubes. *P<0.05, **P<0.01, ***P<0.001; t-tests were used to calculate the p-values.

Journal: International Journal of Biological Sciences

Article Title: MSTN Mutant Promotes Myogenic Differentiation by Increasing Demethylase TET1 Expression via the SMAD2/SMAD3 Pathway

doi: 10.7150/ijbs.40551

Figure Lengend Snippet: Overexpression of TET1 promotes myogenic differentiation. (A-C) The expression of TET1 at the mRNA and protein level after the overexpression of TET1 in the wild type muscle satellite cells. (D, E) Methylation levels of myogenesis-specific genes in the promoter and gene body. (F) Expression of myogenic genes at the mRNA level. (G, H) Expression of myogenic genes at the protein level. (I) The differentiated myotubes. (J) Myotube fusion index after the overexpression of TET1 . (K) Length of myotubes. *P<0.05, **P<0.01, ***P<0.001; t-tests were used to calculate the p-values.

Article Snippet: The TET1 overexpression vector pIRES-hrGFPII-TET1 (#83568) was purchased from Addgene.

Techniques: Over Expression, Expressing, Methylation

Knockdown of TET1 inhibits myogenic differentiation. (A-C) The expression of TET1 at the mRNA and protein level after knockdown of TET1 in MSTN mutant muscle satellite cells. (D, E) Methylation level of myogenesis-specific genes in promoter and gene body. (F) Expression of myogenic genes at the mRNA level. (G, H) Expression of myogenic genes at the protein level. (I) The differentiated myotubes. (J) Myotube fusion index after TET1 knockdown. (K) Length of myotubes. *P<0.05, **P<0.01, ***P<0.001; t-tests were used to calculate the p-values.

Journal: International Journal of Biological Sciences

Article Title: MSTN Mutant Promotes Myogenic Differentiation by Increasing Demethylase TET1 Expression via the SMAD2/SMAD3 Pathway

doi: 10.7150/ijbs.40551

Figure Lengend Snippet: Knockdown of TET1 inhibits myogenic differentiation. (A-C) The expression of TET1 at the mRNA and protein level after knockdown of TET1 in MSTN mutant muscle satellite cells. (D, E) Methylation level of myogenesis-specific genes in promoter and gene body. (F) Expression of myogenic genes at the mRNA level. (G, H) Expression of myogenic genes at the protein level. (I) The differentiated myotubes. (J) Myotube fusion index after TET1 knockdown. (K) Length of myotubes. *P<0.05, **P<0.01, ***P<0.001; t-tests were used to calculate the p-values.

Article Snippet: The TET1 overexpression vector pIRES-hrGFPII-TET1 (#83568) was purchased from Addgene.

Techniques: Knockdown, Expressing, Mutagenesis, Methylation

Schematic of mechanisms for the inhibition of SMAD2/SMAD3 and the activation of TET1 signaling by MSTN mutation. In the wild type model (left), MSTN combined with ActRIIB, followed by the combination of the complex with type I receptor, the activation of the GS region (Ser/Thr) of the receptor, and the transmission of the MSTN signal. The activated MSTN signal then combined and phosphorylated SMAD2/SMAD3, whereby pSMAD2/SMAD3 was activated and combined with SMAD4. This complex entered the nucleus to combine with the promoter of demethylase TET1 , and inhibited the activity of TET1 promoter. In the MSTN mutant model (the right), the MSTN mutant decreased the binding between pSMAD2/SMAD3 and the promoter of TET1 and enhanced TET1 expression, which increased the demethylation of myogenesis-specific genes, including PAX3 , PAX7 , MyoD , and MyoG .

Journal: International Journal of Biological Sciences

Article Title: MSTN Mutant Promotes Myogenic Differentiation by Increasing Demethylase TET1 Expression via the SMAD2/SMAD3 Pathway

doi: 10.7150/ijbs.40551

Figure Lengend Snippet: Schematic of mechanisms for the inhibition of SMAD2/SMAD3 and the activation of TET1 signaling by MSTN mutation. In the wild type model (left), MSTN combined with ActRIIB, followed by the combination of the complex with type I receptor, the activation of the GS region (Ser/Thr) of the receptor, and the transmission of the MSTN signal. The activated MSTN signal then combined and phosphorylated SMAD2/SMAD3, whereby pSMAD2/SMAD3 was activated and combined with SMAD4. This complex entered the nucleus to combine with the promoter of demethylase TET1 , and inhibited the activity of TET1 promoter. In the MSTN mutant model (the right), the MSTN mutant decreased the binding between pSMAD2/SMAD3 and the promoter of TET1 and enhanced TET1 expression, which increased the demethylation of myogenesis-specific genes, including PAX3 , PAX7 , MyoD , and MyoG .

Article Snippet: The TET1 overexpression vector pIRES-hrGFPII-TET1 (#83568) was purchased from Addgene.

Techniques: Inhibition, Activation Assay, Mutagenesis, Transmission Assay, Activity Assay, Binding Assay, Expressing